Synaptic Vesicle Dynamics
Ira Milosevic is now Associate Professor at https://www.ndm.ox.ac.uk/team/ira-milosevic
The laboratory investigates the fundamental aspects of synaptic vesicle recycling related to neurological and neurodegenerative diseases using mouse and mammalian cells as a model system. Cutting edge genomic engineering is combined with the latest imaging and cell biological techniques to study the processes that regulate synaptic vesicle formation. In a distinct but related strand of work, we are exploring the signaling processes that arise from altered neurotransmission and lead to neurodegeneration.
Selected recent publications:
Kondratiuk I, Jakhanwal S, Jin J, Narayanan U, Kroppen B, Krisko A, Meinecke M, Asheri U, Jahn R, Fasshauer D, Milosevic I@ (2020) PI(4,5)P2-dependent regulation of exocytosis by amisyn, a vertebrate-specific competitor of synaptobrevin 2. Proc Natl Acad Sci USA 201908232. doi: 10.1073/pnas.1908232117. Online ahead of print. https://www.pnas.org/content/early/2020/05/27/1908232117.long
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, Maritzen T, Haucke V, Raimundo N, Sørensen JB, Milosevic I@ (2020) Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin. Nat Commun 2020 Mar 9; 11(1):1266. https://www.nature.com/articles/s41467-020-14993-8
Fonseca TB, Sánchez-Guerrero A, Milosevic I@, Raimundo N@ (2019) Mitochondrial fission requires Drp1 but not dynamins. Nature 570(7761):E34-E42, doi: 10.1038/s41586-019-1296-y
Kroll J, Jaime Tobón LM, Vogl C, Neef J, Kondratiuk I, König M, Strenzke N, Wichmann C@, Milosevic I@, Moser T@ (2019) Endophilin-A regulates presynaptic Ca2+ influx and synaptic vesicle recycling in auditory hair cells. EMBO J 38(5). pii: e100116. doi: 10.15252/embj.2018100116.
Yambire KF, Fernandez-Mosquera L, Steinfeld R, Mühle C, Ikonen E, Milosevic I, Raimundo N (2019) Mitochondrial biogenesis is transcriptionally repressed in lysosomal lipid storage diseases. Elife. Feb 18;8. pii: e39598. doi: 10.7554/eLife.39598
Watanabe S, Mamer LE, Raychaudhuri S, Luvsanjav D, Eisen J, Trimbuch T, Söhl-Kielczynski B, Fenske P, Milosevic I, Rosenmund C, Jorgensen EM (2018) Synaptojanin and endophilin mediate neck formation during ultrafast endocytosis. Neuron 98(6), 1184-1197
Farsi Z, Gowrisankaran S, Matija K, Rammner B, Woehler A, Mim C, Jahn R, Milosevic I@ (2018) Clathrin coat controls vesicle acidification by blocking vacuolar ATPase activity. eLife 7, doi: 10.7554/eLife.32569
Fiuza M, Rostosky C, Parkinson G, Bygrave A, Halemani N, Baptista M, Milosevic I, Hanley J (2017) PICK1 regulates AMPA receptor endocytosis via direct interactions with AP2 α-appendage and dynamin. J Cell Biol 216(10), 3323-3338
Murdoch JD, Rostosky C, Gowrisankaran S, Arora AS, Soukup SF, Vidal R, Capece V, Freytag S, Fischer A, Verstreken P, Bonn S, Raimundo N@, Milosevic I@ (2016) Endophilin-A deficiency induces the FoxO3a-Fbxo32 network in the brain and causes dysregulation of autophagy and the ubiquitin-proteasome system. Cell Rep 17(4), 1071-1086
Our work is supported by the German Research Foundation (DFG; Emmy Noether award), Alexander von Humboldt (AvH) Foundation, Schram Foundation, Engelhorn Foundation, SB889 and SFB1190.